The percentage yield of the extract was calculated using the formula below. The medicinal plants are not only used as medicines to maintain their. Phytochemical, proximate, antimicrobial, antioxidant and ftir. The plant extract 50 mg is diluted with distilled water up to 20 ml and this is shaken for 15 minutes in a graduated cylinder. Methanolic extraction of dried leaf along with dry kbr.
The methanol extract of aerial parts were found to be exhibit highest zone of inhibition against s. Fourier transform infrared spectroscopy analysis of. Phytochemical screening and spectroscopic characterization. Typical 1d 1h spectra require less than 10 minutes for data acquisition and analysis. Phytochemical analysis, antibacterial activity, ftir and. Developing ftir microspectroscopy for analysis of plant.
Phytochemical screening by ftir spectroscopic analysis of. Preliminary phytochemical screening, isolation and. Ftir and gcms spectroscopic analysis of methanol and. Green synthesis of silver nanoparticles using the plant. However, the possibilities of ftir analysis go far beyond that. Ftir analysis of methanolic leaf and stem bark extracts confirm the presence of. Green synthesis of silver nanoparticles using aqueous root. Phytochemical screening by ftir spectroscopic analysis of leaf extracts of selected indian medicinal plants r. Phytochemical screening and gcms, ftir analysis of methanolic extract leaves of solanum torvum.
Phytochemical analysis of three extracts showed that the presence of alkaloids, tannins and flavonoids. Identification and quantification of plant extracts using. International journal of health sciences and research. This methanol extract was taken to the ftir and gcms analysis. Medicinal plants are good sources of antioxidants, and. Chromatography and its detection through fourier transform infrared spectroscopy ftir, nuclear magnetic resonance nmr, mass spectrometry ms. Being a native tree of india and malaysia, the plant artocapus heterophyllus was brought into africa by arabs and afterwards. The present study concludes that the ftir analysis reveals clear differentiation between the leaves, flowers and barks extract of e.
Ftir analysis of the methanol extract given the major peak observed. A survey of literature revealed that ftir and uvvis analysis of this medicinal plant was not done so far, thus, the present work has been aimed to produce uvvis and ftir profiles of leaf extract to identify. Phytochemical constituents of the extract were determined. Pdf gcms and ftir analysis on the methanolic extract of. Ftir analysis of the methanol extract given the major.
To evaluate the amount of flavonoids present in the drugs extracts, 0. Ftir measurements were carried out to identify the possible biomolecules in the pedalium murex extract. Ftir studies and spectrophotometric analysis of natural. Multiple compounds can be identified in the same spectrum. Ftir analysis were carried out by shimadzu ftir spectrometer 4000 series, the. In this study, an ecofriendly method was applied for the synthesis of silver nanoparticles ag nps using an aqueous extract of berberis asiatica. Green synthesis of zinc oxide nanoparticles using neem azadirachta indica plant extract dr. Phytochemical and fourier transform infrared spectroscopy. Ftir spectroscopic analysis of leaf extract in hexane in jasminum azoricum l. The comparative account of leaves, barks and flowers.
Uvvis spectra of pure methanolic mentha spicata extract ftir analysis the ftir spectrum was used to identify the functional group of the active components based on the peak value in the. The most methodologically detailed publication on the application of ftirm to residues on stone tools, to date, is that of prinsloo et al. The ftir analysis was used to identify the capping, reducing and stabilizing. Ftir analysis carried out to characterize the agnps obtained from each type of plant extract curve a, banana. In vitro antiinflammatory, anticancer mcf7, 3t3, and. Polyphenols have previously been suggested to be involved in the plant extract mediated aunps synthesis on the bases of the ftir data song et al. Fourier transform infrared ftir spectroscopic analysis of nicotiana. The present investigation focus on screening of phytochemicals and ftir analysis of. Preliminary phytochemical analysis was carried out to identify the phytoconstituents present in the extract of c. Fourier transform infrared spectroscopy analysis of erythrina. Preparation and characterization of gold nanoparticles. In ftir analysis, the extract and fractions of the plant with the concentration of 10 mg were encapsulated in the pellet of 100 mg of. Agnps obtained showed significantly higher antimicrobial activities against escherichia coli e. The extract and fractions of the plant were dried for the analysis of ftir.
Flavonoid was extracted from medicinal plant leaves by. Ftir spectrum analysis of aqueous extract of erythrina variegata l. The ftir analysis of leaf powder of the plant revealed the following functional. These authors generated absorbance spectra for a range of residue types including bone, muscle, and adipose fat using ftir. Pdf ftir spectroscopic analysis of leaf extract in hexane in. Methanol extract of both the plants was found to possess highest antioxidant potential in comparison. Ramamoorthi and kannan 2007 screened the bioactive group of chemicals in the dry leaf powder of calotropis gigantea by ftir analysis.
Infrared ir spectroscopy combined with chemometrics has been developed for simple analysis of flavonoid in the medicinal plant extract. So, the present study concluded that the flower extract possessed strong functional groups, when. Fourier transform infrared ftir analysis the ftir spectrum was used to identify the functional groups in the plant extract responsible for the reduction of silver ions for the synthesis of silver. The present study was carried out to characterize the bioactive constituents present in aerial parts of ethanolic extract of sarcostemma brevistigma using uvvis and ftir. Research article analysis of flavonoid in medicinal plant. Ftir spectroscopic analysis of leaf extract in hexane in jasminum azoricum l article pdf available january 2019 with 609 reads how we measure reads. Green synthesis of zinc oxide nanoparticles using neem.
Pdf analysis of flavonoid in medicinal plant extract. Ftir analysis the ftir measurement of plant extract and copper nanoparticles are shown in fig 2a and2b, respectively. Pdf ftir spectroscopic analysis of leaf extract in. Pdf analysis of flower extract of michelia champaca l. Analysis of flavonoid in medicinal plant extract using infrared spectroscopy and chemometrics. The formation of 2 cm thick foam indicates the presence of saponins 3. The ftir analysis of the whole plant of andrographis lineata confirmed the of alkyl halides, alkenes, acyl or nitro compounds, ethers or alkoxy, esters, alkanes or aromatics, aromatic ring, alkene. The phytochemical analysis and ftir fourier transform. Ftir analysis the identifications of the active functional groups presence in inoculated aquilaria malaccensis oil, hydrosol and leaves extracts was conducted by the aid of ftir spectroscopy perkin.
Ramaswamy department of zoology, karpagam university, coimbatore. Functional group analysis of moringa concanensis nimmo. Analysis of flavonoid in medicinal plant extract using. Ftir spectrophotometer was performed to assess the functional groups of the leaf extract. Research article gcms analysis of phytochemical compounds present in the rhizomes of nervilia aragoana gaud elizabeth thomas1, aneesh t. Green synthesis of silver nanoparticles from the leaf. Ftir spectra of dried aqueous extract and synthesised agnps are shown in. Pdf phytochemical analysis of mentha spicata plant.
Ftir analysis for five selected green leafy vegetables glvs viz. The leaf extract obtained at the flask finally collected after the completion of soxhlation. The ftir spectra of fresh tulsi extract showed peaks at around 1636, 22 and 3336 cm. The powdered sample of each plant specimen was loaded in ftir spectroscope shimadzu, ir affinity1, japan, with a scan range from 400 to 4000 cm 1 with a resolution of 4cm 1. Phytochemical analysis was carried out by using ftir spectroscopy and gcms analysis. Phytochemical screening and gcms, ftir analysis of.
A survey of literature revealed that the ftir analysis of functional groups was not done so far. The equipment used was perkin elmer spectrum gx, 16 scans per samples in the region of 4000650 cm 1. Phytochemical screening by ftir spectroscopic analysis of leaf. Fourier transform infrared spectroscopy ftir analysis. Vitis vinifera seed is a member of vitaceae family which contains high phenolic procyanidins, gallic acid, catechin, anthocyanin, vitamin e, linoleic acid and flavonoids. Ftir spectroscopy allows a reliable distinction between thcs and cbds hemp within a few seconds. Ftir analysis and quantification of phenols and flavonoids. Microorganisms, whole plants, plant tissue and fruits, plant extracts and marine algae have been used to produce nanoparticles 49. Furthermore,5 have shown that seed aqueous extract possessed mild. Antibacterial and ftir spectral analysis of methanolic extract of gliricidia sepium leaves m. Ftir spectrum analysis of ethanolic extract of erythrina variegata l. Functional group analysis of moringa concanensis nimmo moringaceae by ftir spectrum 29 ii.
Infra red spectra of methanol extract of medicinal plant michelia champaca linn flowers were recorded. Ftir and gcms analysis of bioactive phytocompounds in. Research article using ftir, hplc and gcms analysis. Phytochemical, proximate, antimicrobial, antioxidant and.
Plant extract mediated synthesis of metal nanoparticles has an edge over. Phytochemical analysis of mentha spicata plant extract using uvvis, ftir and gcms technique article pdf available in journal of chemical and pharmaceutical research 201682. Antibacterial and ftir spectral analysis of methanolic. The vibrational assignments, intensities and wave number cm1 of dominant peak were obtained from absorption spectra.
97 1554 280 1185 1153 1238 1315 1081 952 937 1321 57 657 772 1105 1064 400 1016 310 487 268 352 816 1139 483 695 249 216 250 1401 1070 419 1265